{"id":131593,"date":"2021-09-04T17:14:17","date_gmt":"2021-09-04T11:44:17","guid":{"rendered":"https:\/\/blog.forumias.com\/?page_id=131593"},"modified":"2021-09-04T17:14:17","modified_gmt":"2021-09-04T11:44:17","slug":"how-the-genome-sequencing-works","status":"publish","type":"page","link":"https:\/\/forumias.com\/blog\/how-the-genome-sequencing-works\/","title":{"rendered":"How the Genome sequencing works?\u00a0"},"content":{"rendered":"<p><span data-contrast=\"auto\"><strong>Firstly,<\/strong> the RNA of the virus sample is converted to DNA. It is cut into short fragments. Several copies of each fragment are made.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\"><strong>Secondly,<\/strong> these fragments are loaded into a sequencer machine. The machine &#8216;reads&#8217; the order of nucleotides of each of those several segments. The nucleotides are designated English alphabets A, T, C, G in a certain order.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\"><strong>Thirdly,<\/strong> these \u2018reads\u2019 of several individual small segments are then fed into computer software. The software puts all those \u2018reads\u2019 together to form one complete genome or a whole genome sequence of nucleotides. This completes sequencing.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><span data-contrast=\"auto\"><strong>Finally,<\/strong> the genome, which has been sequenced, is assigned a lineage through other web-based programmes. These lineages help one determine what variant it is, whether it matches with the lineage of nationally or internationally existing variants of the virus or a new variant has come up<\/span><b><span data-contrast=\"auto\">.\u00a0<\/span><\/b><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Firstly, the RNA of the virus sample is converted to DNA. It is cut into short fragments. Several copies of each fragment are made.\u00a0 Secondly, these fragments are loaded into a sequencer machine. The machine &#8216;reads&#8217; the order of nucleotides of each of those several segments. The nucleotides are designated English alphabets A, T, C,&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/how-the-genome-sequencing-works\/\">Continue reading <span class=\"screen-reader-text\">How the Genome sequencing works?\u00a0<\/span><\/a><\/p>\n","protected":false},"author":10328,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-131593","page","type-page","status-publish","hentry","entry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/pages\/131593","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/10328"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=131593"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/pages\/131593\/revisions"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=131593"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}