{"id":333210,"date":"2025-04-17T12:21:38","date_gmt":"2025-04-17T06:51:38","guid":{"rendered":"https:\/\/forumias.com\/blog\/?p=333210"},"modified":"2025-04-18T19:55:17","modified_gmt":"2025-04-18T14:25:17","slug":"rt-lamp-assay-for-tuberculosis-diagnosis","status":"publish","type":"post","link":"https:\/\/forumias.com\/blog\/rt-lamp-assay-for-tuberculosis-diagnosis\/","title":{"rendered":"rt-LAMP Assay for Tuberculosis Diagnosis"},"content":{"rendered":"<p><strong>News- <\/strong>Researchers have evaluated the real-time Loop-Mediated Isothermal Amplification (rt-LAMP) method for the early and accurate detection of tuberculosis (TB). <strong>rt-LAMP Assay for Tuberculosis Diagnosis.<\/strong><\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\" wp-image-333449 aligncenter\" src=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?resize=490%2C325&#038;ssl=1\" alt=\"rt-LAMP Assay for Tuberculosis Diagnosis\" width=\"490\" height=\"325\" srcset=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?resize=300%2C199&amp;ssl=1 300w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?resize=1024%2C680&amp;ssl=1 1024w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?resize=768%2C510&amp;ssl=1 768w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?w=1280&amp;ssl=1 1280w\" sizes=\"auto, (max-width: 490px) 100vw, 490px\" \/><\/p>\n<h2>About rt-LAMP Assay<\/h2>\n<ul>\n<li>rt-LAMP (Real-time Loop-mediated Isothermal Amplification) is a molecular diagnostic test that <em>enables the rapid detection of TB DNA.<\/em><\/li>\n<li>It uses a one-step DNA amplification process, capable of detecting TB DNA with as few as <em>10 copies per microliter in a sample<\/em>.<\/li>\n<li>The <em>test functions at a single temperature<\/em>, unlike traditional RT-PCR which requires three different temperature cycles.<\/li>\n<li>The rt-LAMP assay has been <em>approved by the Central Drugs Standard Control Organisation (CDSCO)<\/em> and is currently awaiting validation from the Indian Council of Medical Research (ICMR).<\/li>\n<\/ul>\n<p><strong>Key Features:<\/strong><\/p>\n<ul>\n<li><strong>Open Platform<\/strong>: The rt-LAMP assay is designed as an <em>open platform system<\/em>, which allows existing RT-PCR machines to be repurposed for TB diagnosis by reprogramming them.<\/li>\n<li><strong>Primer Design<\/strong>: It <em>uses six primers<\/em>, significantly more than the two primers used in RT-PCR, enhancing its efficiency and specificity.<\/li>\n<li><strong>Rapid Results<\/strong>: Thanks to its high amplification rate, <em>positive results can be obtained in just 10\u201320 minutes.<\/em><\/li>\n<li><strong>Low Cost:<\/strong> The test uses inexpensive dyes like Syto 16 (a fluorescent dye) and affordable primers, making it cost-effective.<\/li>\n<li><strong>High throughput capability<\/strong>: The rt-LAMP system can process up to 96 or even 384 patient samples in a single run, making it <em>suitable for mass screening<\/em>.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>News- Researchers have evaluated the real-time Loop-Mediated Isothermal Amplification (rt-LAMP) method for the early and accurate detection of tuberculosis (TB). rt-LAMP Assay for Tuberculosis Diagnosis. About rt-LAMP Assay rt-LAMP (Real-time Loop-mediated Isothermal Amplification) is a molecular diagnostic test that enables the rapid detection of TB DNA. It uses a one-step DNA amplification process, capable of&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/rt-lamp-assay-for-tuberculosis-diagnosis\/\">Continue reading <span class=\"screen-reader-text\">rt-LAMP Assay for Tuberculosis Diagnosis<\/span><\/a><\/p>\n","protected":false},"author":10367,"featured_media":333449,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"categories":[1566,1738,12039],"tags":[11872],"class_list":["post-333210","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-daily-factly-articles","category-science-and-technology-daily-factly-articles","category-knolls","tag-9pm-daily-factly","entry"],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2025\/04\/rt-LAMP-Assay-for-Tuberculosis-Diagnosis.png?fit=1280%2C850&ssl=1","views":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/333210","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/10367"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=333210"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/333210\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media\/333449"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=333210"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/categories?post=333210"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/tags?post=333210"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}