{"id":351853,"date":"2025-12-12T14:17:51","date_gmt":"2025-12-12T08:47:51","guid":{"rendered":"https:\/\/forumias.com\/blog\/?p=351853"},"modified":"2025-12-12T14:17:51","modified_gmt":"2025-12-12T08:47:51","slug":"glowcas9","status":"publish","type":"post","link":"https:\/\/forumias.com\/blog\/glowcas9\/","title":{"rendered":"GlowCas9"},"content":{"rendered":"<p><strong>News:<\/strong> Indian scientists have created a glowing CRISPR protein that can be observed in action within living cells. It is a groundbreaking development that could greatly enhance cancer treatment and other gene-based therapies.<\/p>\n<h2><strong>About GlowCas9<\/strong><\/h2>\n<p><img data-recalc-dims=\"1\" decoding=\"async\" class=\"aligncenter\" src=\"https:\/\/i0.wp.com\/dst.gov.in\/sites\/default\/files\/GlowCas9.png?w=750&#038;ssl=1\" alt=\"GlowCas9\" \/><\/p>\n<ul>\n<li>GlowCas9 is a new CRISPR protein created by <strong>scientists at the Bose Institute in Kolkata.<\/strong><\/li>\n<li>It is engineered <strong>to produce light during gene editing<\/strong>, enabling scientists to track the process inside living cells.<\/li>\n<li><strong>Mechanism of Action:<\/strong>\n<ul>\n<li>It is a <strong>bioluminescent version of the Cas9 protein,<\/strong> developed by fusing Cas9 with <strong>a split nano-luciferase enzyme derived from deep-sea shrimp.\u00a0<\/strong><\/li>\n<li>These <strong>nano-luciferase enzyme<\/strong> pieces are inactive until Cas9 folds correctly.<\/li>\n<li>When the pieces come into proximity, they reassemble, restoring enzymatic activity and producing a visible light signal, similar to the glow of fireflies.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Advantages of GlowCas9:<\/strong>\n<ul>\n<li>It provides a way <strong>to observe gene editing in real-time without harming cells.<\/strong><\/li>\n<li>The<strong> bioluminescence allows tracking of the gene-editing process in living cells, tissues, and even plant leaves.<\/strong><\/li>\n<li>GlowCas9 is<strong> more stable than conventional Cas9<\/strong> and <strong>can maintain its structure and activity<\/strong> at higher temperatures. This increased stability is important for gene therapy, ensuring effective delivery of the Cas9 protein for treatment.<\/li>\n<\/ul>\n<\/li>\n<li><b>Application:<\/b>\n<ul>\n<li><b><\/b><strong>Gene Therapy Implications: <\/strong>GlowCas9 can aid in gene therapy <strong>by improving the precision of homology-directed repair (HDR),<\/strong> which is essential for fixing hereditary mutations linked to diseases like sickle cell anemia and muscular dystrophy.<\/li>\n<li><strong>Applications in Crop Improvement: <\/strong>The technology is<strong> also applicable to plant systems, suggesting potential non-transgenic applications in crop improvement.<\/strong><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>News: Indian scientists have created a glowing CRISPR protein that can be observed in action within living cells. It is a groundbreaking development that could greatly enhance cancer treatment and other gene-based therapies. About GlowCas9 GlowCas9 is a new CRISPR protein created by scientists at the Bose Institute in Kolkata. It is engineered to produce&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/glowcas9\/\">Continue reading <span class=\"screen-reader-text\">GlowCas9<\/span><\/a><\/p>\n","protected":false},"author":10366,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"categories":[1566,1738,12039],"tags":[11872,12162],"class_list":["post-351853","post","type-post","status-publish","format-standard","hentry","category-daily-factly-articles","category-science-and-technology-daily-factly-articles","category-knolls","tag-9pm-daily-factly","tag-madhyamamonline-com","entry"],"jetpack_featured_media_url":"","views":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/351853","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/10366"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=351853"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/351853\/revisions"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=351853"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/categories?post=351853"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/tags?post=351853"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}