{"id":356333,"date":"2026-02-18T15:25:34","date_gmt":"2026-02-18T09:55:34","guid":{"rendered":"https:\/\/forumias.com\/blog\/?p=356333"},"modified":"2026-02-18T15:26:12","modified_gmt":"2026-02-18T09:56:12","slug":"prime-editing-mediated-readthrough-of-premature-termination-codons-pert","status":"publish","type":"post","link":"https:\/\/forumias.com\/blog\/prime-editing-mediated-readthrough-of-premature-termination-codons-pert\/","title":{"rendered":"Prime-Editing-mediated Readthrough of premature Termination codons (PERT)"},"content":{"rendered":"<p><strong>News: <\/strong>Researchers from the Broad Institute recently reported a new gene-editing strategy called Prime Editing\u2013Mediated Readthrough of Premature Termination Codons (PERT) that may enable a single treatment to address many genetic diseases caused by nonsense mutations.<\/p>\n<h2 style=\"text-align: justify;\"><strong>About Prime Editing\u2013Mediated Readthrough of Premature Termination Codons (PERT)<\/strong><\/h2>\n<figure id=\"attachment_356334\" aria-describedby=\"caption-attachment-356334\" style=\"width: 435px\" class=\"wp-caption aligncenter\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\" wp-image-356334\" src=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/02\/1280.jpg?resize=435%2C299&#038;ssl=1\" alt=\"\" width=\"435\" height=\"299\" srcset=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/02\/1280.jpg?resize=300%2C206&amp;ssl=1 300w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/02\/1280.jpg?resize=768%2C527&amp;ssl=1 768w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/02\/1280.jpg?w=855&amp;ssl=1 855w\" sizes=\"auto, (max-width: 435px) 100vw, 435px\" \/><figcaption id=\"caption-attachment-356334\" class=\"wp-caption-text\">Source: dcard<\/figcaption><\/figure>\n<div class=\"flex flex-col text-sm pb-25\">\n<article class=\"text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [&amp;:has([data-writing-block])&gt;*]:pointer-events-auto scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]\" dir=\"auto\" tabindex=\"-1\" data-turn-id=\"request-WEB:235befc0-52d3-4a4a-80d2-a4dc9afffeb1-4\" data-testid=\"conversation-turn-10\" data-scroll-anchor=\"true\" data-turn=\"assistant\">\n<div class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:--spacing(4)] @w-sm\/main:[--thread-content-margin:--spacing(6)] @w-lg\/main:[--thread-content-margin:--spacing(16)] px-(--thread-content-margin)\">\n<div class=\"[--thread-content-max-width:40rem] @w-lg\/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\" tabindex=\"-1\">\n<div class=\"flex max-w-full flex-col grow\">\n<div class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal [.text-message+&amp;]:mt-1\" dir=\"auto\" data-message-author-role=\"assistant\" data-message-id=\"0a361c9a-0528-4843-a127-2205d060e449\" data-message-model-slug=\"gpt-5-2\">\n<div class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[1px]\">\n<div class=\"markdown prose dark:prose-invert w-full wrap-break-word light markdown-new-styling\">\n<ul>\n<li data-start=\"0\" data-end=\"170\">PERT is a genome-editing strategy designed to treat genetic diseases caused by nonsense mutations, which prematurely halt protein production.<\/li>\n<li data-start=\"172\" data-end=\"403\"><strong data-start=\"172\" data-end=\"193\">Problem addressed<\/strong> \u2013 Nonsense mutations introduce premature termination codons (PTCs) in mRNA, causing early stoppage of translation and resulting in incomplete, nonfunctional proteins responsible for many inherited disorders.<\/li>\n<li data-start=\"405\" data-end=\"601\"><strong data-start=\"405\" data-end=\"429\">Core technology used<\/strong> \u2013 PERT is based on prime editing, an advanced CRISPR\/Cas9-based system that works as a precise \u201csearch-and-replace\u201d tool and does not create double-strand breaks in DNA.<\/li>\n<li data-start=\"603\" data-end=\"767\"><strong data-start=\"603\" data-end=\"620\">Key mechanism<\/strong> \u2013 PERT does not directly correct the disease-causing mutation. Instead, it inserts an engineered suppressor transfer RNA (tRNA) into the genome.<\/li>\n<li data-start=\"769\" data-end=\"1000\"><strong data-start=\"769\" data-end=\"796\">Role of suppressor tRNA<\/strong> \u2013 The engineered tRNA recognizes premature stop codons and inserts an amino acid in place of the stop signal, enabling the ribosome to continue translation and produce a full-length functional protein.<\/li>\n<li data-start=\"1002\" data-end=\"1216\"><strong data-start=\"1002\" data-end=\"1020\">Unique feature<\/strong> \u2013 The suppressor tRNA is inserted at a redundant tRNA gene site, ensuring stable expression without disrupting essential genes, and making the strategy independent of the specific mutated gene.<\/li>\n<li data-start=\"1218\" data-end=\"1560\"><strong data-start=\"1218\" data-end=\"1245\">Advantages &amp; Limitation<\/strong> \u2013 A single PERT-based therapy could potentially treat multiple unrelated genetic diseases caused by nonsense mutations, reducing time and cost of drug development; however, the technology is still in early research stages and requires further animal and clinical testing to establish safety and long-term efficacy.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>News: Researchers from the Broad Institute recently reported a new gene-editing strategy called Prime Editing\u2013Mediated Readthrough of Premature Termination Codons (PERT) that may enable a single treatment to address many genetic diseases caused by nonsense mutations. About Prime Editing\u2013Mediated Readthrough of Premature Termination Codons (PERT) PERT is a genome-editing strategy designed to treat genetic diseases&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/prime-editing-mediated-readthrough-of-premature-termination-codons-pert\/\">Continue reading <span class=\"screen-reader-text\">Prime-Editing-mediated Readthrough of premature Termination codons (PERT)<\/span><\/a><\/p>\n","protected":false},"author":10366,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"categories":[1566,1738,12039],"tags":[11872,10498],"class_list":["post-356333","post","type-post","status-publish","format-standard","hentry","category-daily-factly-articles","category-science-and-technology-daily-factly-articles","category-knolls","tag-9pm-daily-factly","tag-the-hindu","entry"],"jetpack_featured_media_url":"","views":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/356333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/10366"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=356333"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/356333\/revisions"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=356333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/categories?post=356333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/tags?post=356333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}