{"id":363756,"date":"2026-05-27T16:48:22","date_gmt":"2026-05-27T11:18:22","guid":{"rendered":"https:\/\/forumias.com\/blog\/?p=363756"},"modified":"2026-05-27T16:48:22","modified_gmt":"2026-05-27T11:18:22","slug":"cleavable-light-erased-antibody-reporter-clear-platform","status":"publish","type":"post","link":"https:\/\/forumias.com\/blog\/cleavable-light-erased-antibody-reporter-clear-platform\/","title":{"rendered":"Cleavable Light-Erased Antibody Reporter (CLEAR) Platform"},"content":{"rendered":"<div class=\"content-box-green\">\n<p><strong>News:<\/strong> Researchers at the Jawaharlal Nehru Centre for Advanced Scientific Research developed the CLEAR platform for high-resolution protein mapping.<\/p>\n<\/div>\n<h2 class=\"red-h2-box\"><strong>About Cleavable Light-Erased Antibody Reporter (CLEAR) Platform<\/strong><\/h2>\n<figure id=\"attachment_363758\" aria-describedby=\"caption-attachment-363758\" style=\"width: 684px\" class=\"wp-caption aligncenter\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\" wp-image-363758\" src=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/05\/Source-DST.jpg?resize=684%2C269&#038;ssl=1\" alt=\"Cleavable Light-Erased Antibody Reporter (CLEAR) Platform\" width=\"684\" height=\"269\" srcset=\"https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/05\/Source-DST.jpg?resize=300%2C118&amp;ssl=1 300w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/05\/Source-DST.jpg?resize=768%2C303&amp;ssl=1 768w, https:\/\/i0.wp.com\/forumias.com\/blog\/wp-content\/uploads\/2026\/05\/Source-DST.jpg?w=875&amp;ssl=1 875w\" sizes=\"auto, (max-width: 684px) 100vw, 684px\" \/><figcaption id=\"caption-attachment-363758\" class=\"wp-caption-text\">Source &#8211; DST<\/figcaption><\/figure>\n<ul>\n<li><strong>Cleavable Light-Erased Antibody Reporter (CLEAR):<\/strong> It is a novel <strong>spatial protein imaging platform<\/strong> that <strong>enables visualization and mapping of a large number of proteins within the same sample.<\/strong><\/li>\n<li><strong>Developed by:<\/strong> The platform was developed by researchers at <strong>Jawaharlal Nehru Centre for Advanced Scientific Research<\/strong>, an autonomous institute of the <strong>Department of Science and Technology (DST).<\/strong><\/li>\n<li><strong>Working Mechanism:<\/strong>\n<ul>\n<li><strong>Targeted Labelling:<\/strong> Scientists use CLEAR <strong>probes containing antibodies linked with fluorescent tags through a light-sensitive chemical bond to label proteins.<\/strong><\/li>\n<li><strong>Image Capture:<\/strong> The labelled proteins are imaged under a microscope within the same spectral window.<\/li>\n<li><strong>Photolytic Erasing:<\/strong> A <strong>gentle pulse of 365 nm LED light removes the fluorescent signal<\/strong> by cleaving the chemical bond without damaging the sample.<\/li>\n<li><strong>Cyclic Rewriting:<\/strong> A <strong>new set of proteins is labelled and imaged repeatedly in the same optical window<\/strong> to generate detailed protein maps.<\/li>\n<li><strong>Detailed Mapping:<\/strong> Repeated imaging cycles create information-rich maps across cells and tissue sections.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Key Features of CLEAR:<\/strong>\n<ul>\n<li><strong>High-Plex Multiplexing:<\/strong> It enables visualization of a very large number of proteins within a single biological sample.<\/li>\n<li><strong>Live-Cell Compatibility:<\/strong> The gentle <strong>light-erasing process preserves delicate biological samples, including live cells.<\/strong><\/li>\n<li><strong>High Spatial Resolution:<\/strong> It captures the precise spatial arrangement of proteins within tissues.<\/li>\n<li><strong>Speed and Simplified Imaging:<\/strong> The platform repeatedly uses a single fluorescent channel and reduces dependence on complex multi-laser imaging systems.<\/li>\n<li><strong>Chalkboard-Like Imaging: <\/strong>The platform repeatedly erases and rewrites fluorescent signals like a chalkboard using a single fluorophore.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Significance<\/strong>: CLEAR can improve <strong>disease diagnosis, biomedical research, and precision medicine<\/strong> through comprehensive high-resolution protein mapping.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>News: Researchers at the Jawaharlal Nehru Centre for Advanced Scientific Research developed the CLEAR platform for high-resolution protein mapping. About Cleavable Light-Erased Antibody Reporter (CLEAR) Platform Cleavable Light-Erased Antibody Reporter (CLEAR): It is a novel spatial protein imaging platform that enables visualization and mapping of a large number of proteins within the same sample. Developed&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/cleavable-light-erased-antibody-reporter-clear-platform\/\">Continue reading <span class=\"screen-reader-text\">Cleavable Light-Erased Antibody Reporter (CLEAR) Platform<\/span><\/a><\/p>\n","protected":false},"author":10401,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"categories":[1566,1738,12039],"tags":[11872],"class_list":["post-363756","post","type-post","status-publish","format-standard","hentry","category-daily-factly-articles","category-science-and-technology-daily-factly-articles","category-knolls","tag-9pm-daily-factly","entry"],"jetpack_featured_media_url":"","views":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/363756","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/10401"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=363756"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/363756\/revisions"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=363756"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/categories?post=363756"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/tags?post=363756"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}