{"id":85479,"date":"2020-03-18T14:42:11","date_gmt":"2020-03-18T09:12:11","guid":{"rendered":"http:\/\/factly.forumias.com\/?p=14312"},"modified":"2020-03-18T14:42:11","modified_gmt":"2020-03-18T09:12:11","slug":"polymerase-chain-reactionpcr","status":"publish","type":"post","link":"https:\/\/forumias.com\/blog\/polymerase-chain-reactionpcr\/","title":{"rendered":"Polymerase chain reaction(PCR)"},"content":{"rendered":"\n<p><strong>News:<\/strong>The Indian Council of Medical Research(ICMR) has said the designated labs will use the conventional real-time polymerase chain reaction(PCR) test to test for the novel coronavirus that causes COVID-19.<br><\/p>\n\n\n\n<p><strong>Facts:<\/strong><br><\/p>\n\n\n\n<p><strong>About Polymerase chain reaction(PCR):<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>The polymerase chain reaction(PCR) is a technique which makes numerous copies of a specific segment of DNA quickly and accurately.&nbsp;<\/li><li>It was developed by the American biochemist Kary Mullis in 1983.He was awarded the Nobel Prize in Chemistry in 1993 for his pioneering work.<\/li><li>This technique enables investigators to obtain the large quantities of DNA that are required for various experiments and procedures in molecular biology, forensic analysis and medical diagnostics.<\/li><\/ul>\n\n\n\n<p><strong>About PCR Test:<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>PCR test is conducted on a swab collected from the back of the throat, a liquid sample from the lower respiratory tract or a simple saliva sample. Such tests are commonly used in Influenza A, Influenza B and H1N1 virus detection.<\/li><li>Since,SARS-COV-2 is a virus made of RNA which needs to be converted into DNA.For this,the technique includes a process called reverse transcription.&nbsp;<\/li><li>A \u2018reverse transcriptase\u2019 enzyme converts the RNA into DNA.Copies of the DNA are then made and amplified.A fluorescent DNA binding dye called the probe shows the presence of the virus.<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>News:The Indian Council of Medical Research(ICMR) has said the designated labs will use the conventional real-time polymerase chain reaction(PCR) test to test for the novel coronavirus that causes COVID-19. Facts: About Polymerase chain reaction(PCR): The polymerase chain reaction(PCR) is a technique which makes numerous copies of a specific segment of DNA quickly and accurately.&nbsp; It&hellip; <a class=\"more-link\" href=\"https:\/\/forumias.com\/blog\/polymerase-chain-reactionpcr\/\">Continue reading <span class=\"screen-reader-text\">Polymerase chain reaction(PCR)<\/span><\/a><\/p>\n","protected":false},"author":61,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"categories":[1738],"tags":[2834],"class_list":["post-85479","post","type-post","status-publish","format-standard","hentry","category-science-and-technology-daily-factly-articles","tag-polymerase-chain-reaction","entry"],"jetpack_featured_media_url":"","views":{"total":0,"cached_at":"","cached_date":1704887140},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/85479","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/users\/61"}],"replies":[{"embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/comments?post=85479"}],"version-history":[{"count":0,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/posts\/85479\/revisions"}],"wp:attachment":[{"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/media?parent=85479"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/categories?post=85479"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/forumias.com\/blog\/wp-json\/wp\/v2\/tags?post=85479"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}