Doubt Clearance Thread: UPSC 2021
"When in doubt, observe and ask questions. When certain, observe at length and ask many more questions."
Created this thread as a one stop solution for all members so that all the doubts wherein any conceptual clarification is required can be solved here.
How does DNA vaccine work for COVID?DNA vaccine uses a gene (the gene that is responsible for the spike protein of COVID) from the virus and once it enters the nucleus it is transcribed into mRNA and then translated into the spike protein. Now our bodies will create antibodies to that protein. In mRNA that first step of transcribing into mRNA is skipped.
But Corona virus is a RNA virus and not a DNA virus. Then how come DNA is made from RNA?
mRNA vaccine could be understood but not DNA. If I am worng or I am missing something then pls tell.
Background Info:
Dna->is a piece of genetic info.
Spike protein->It's the part of coronavirus which latches to cells.
mRNA->used for sending the info to the Ribosomes which synthesizes the spike protein. (Because DNA ki bhasha ribosome ko nhi aati.)
Application:
So, in this vaccine, we are just sending the genetic information, not the actual virus. Now we can send the genetic info in rna or DNA form doesn't matter. Dna enters our nucleus forms the mrna which goes to the ribosomes which makes the spike protein, which in turns activates our immune system and creates antibodies.
Found this vedio. It says the RNA from the virus is attached with a DNA from a virus which is not harmful and then in some manner the DNA is formed which then is inserted into the cells - through different methods.
captainhaddock,
How does DNA vaccine work for COVID?DNA vaccine uses a gene (the gene that is responsible for the spike protein of COVID) from the virus and once it enters the nucleus it is transcribed into mRNA and then translated into the spike protein. Now our bodies will create antibodies to that protein. In mRNA that first step of transcribing into mRNA is skipped.
But Corona virus is a RNA virus and not a DNA virus. Then how come DNA is made from RNA?
mRNA vaccine could be understood but not DNA. If I am worng or I am missing something then pls tell.
Background Info:
Dna->is a piece of genetic info.
Spike protein->It's the part of coronavirus which latches to cells.
mRNA->used for sending the info to the Ribosomes which synthesizes the spike protein. (Because DNA ki bhasha ribosome ko nhi aati.)
Application:
So, in this vaccine, we are just sending the genetic information, not the actual virus. Now we can send the genetic info in rna or DNA form doesn't matter. Dna enters our nucleus forms the mrna which goes to the ribosomes which makes the spike protein, which in turns activates our immune system and creates antibodies.
Found this vedio. It says the RNA from the virus is attached with a DNA from a virus which is not harmful and then in some manner the DNA is formed which then is inserted into the cells - through different methods.
Bro how can Rna be attached to DNA? Rna is single-stranded, DNA is double.Yha vo Rna ko double stranded dikha rha hai means he might have converted RNA into DNA ( by reverse transcriptome).
Bro how can Rna be attached to DNA? Rna is single-stranded, DNA is double.Yha vo Rna ko double stranded dikha rha hai means he might have converted RNA into DNA ( by reverse transcriptome).
Yeah! Very much possible. I also couldn't understand that. But I was no where getting that I thought yeh hoga shayad - that's why wrote in some manner DNA might be formed. No more hair-splitting.
I too find Reverse transcriptome method to be the right explanation for conversion of RNA into DNA.
Bro how can Rna be attached to DNA? Rna is single-stranded, DNA is double.Yha vo Rna ko double stranded dikha rha hai means he might have converted RNA into DNA ( by reverse transcriptome).
RNA with its complimentary bases makes up the DNA with similar genetic info carrier. Both vaccines work on similar mechanism, just the carriers of genetic info of virus antigens are different i.e. RNA and DNA
How does DNA vaccine work for COVID?DNA vaccine uses a gene (the gene that is responsible for the spike protein of COVID) from the virus and once it enters the nucleus it is transcribed into mRNA and then translated into the spike protein. Now our bodies will create antibodies to that protein. In mRNA that first step of transcribing into mRNA is skipped.
But Corona virus is a RNA virus and not a DNA virus. Then how come DNA is made from RNA?
mRNA vaccine could be understood but not DNA. If I am worng or I am missing something then pls tell.
Vision me diya hai ki some plasmid DNA from a harmless bacteria is used to deliver info to the cells to teach them to form spike proteins. Bas ratt lia
How does DNA vaccine work for COVID?DNA vaccine uses a gene (the gene that is responsible for the spike protein of COVID) from the virus and once it enters the nucleus it is transcribed into mRNA and then translated into the spike protein. Now our bodies will create antibodies to that protein. In mRNA that first step of transcribing into mRNA is skipped.
But Corona virus is a RNA virus and not a DNA virus. Then how come DNA is made from RNA?
mRNA vaccine could be understood but not DNA. If I am worng or I am missing something then pls tell.
Vision me diya hai ki some plasmid DNA from a harmless bacteria is used to deliver info to the cells to teach them to form spike proteins. Bas ratt lia
Science mei shayad yehi karna chayeih! I was hell bent on understanding :/
sstarrr,if everybody is talking about COVID related stuff
also look forNanopore sequencingin brief
Nanopore sequencing is a third generation approach used in the sequencing of biopolymers- specifically, polynucleotides in the form of DNA or RNA. Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced withoutthe need for PCR amplification or chemical labeling of the sample.
SC in their judgement said they can also punish for contempt of HC, subordi, tribunals
mehuifs,Shailputri@Hououin_Kyouma lol no, bacche parents ki jagah kaise le lenge
bacche can be like, aise kaise hamarey parents ko bol diya :P
captainhaddock,
@Hououin_Kyouma lol no, baccha baap ki jagah kaise le lega
HC flexing its writ petition powers :p .
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