News: Indian scientists have created a glowing CRISPR protein that can be observed in action within living cells. It is a groundbreaking development that could greatly enhance cancer treatment and other gene-based therapies.
About GlowCas9
- GlowCas9 is a new CRISPR protein created by scientists at the Bose Institute in Kolkata.
- It is engineered to produce light during gene editing, enabling scientists to track the process inside living cells.
- Mechanism of Action:
- It is a bioluminescent version of the Cas9 protein, developed by fusing Cas9 with a split nano-luciferase enzyme derived from deep-sea shrimp.
- These nano-luciferase enzyme pieces are inactive until Cas9 folds correctly.
- When the pieces come into proximity, they reassemble, restoring enzymatic activity and producing a visible light signal, similar to the glow of fireflies.
- Advantages of GlowCas9:
- It provides a way to observe gene editing in real-time without harming cells.
- The bioluminescence allows tracking of the gene-editing process in living cells, tissues, and even plant leaves.
- GlowCas9 is more stable than conventional Cas9 and can maintain its structure and activity at higher temperatures. This increased stability is important for gene therapy, ensuring effective delivery of the Cas9 protein for treatment.
- Application:
- Gene Therapy Implications: GlowCas9 can aid in gene therapy by improving the precision of homology-directed repair (HDR), which is essential for fixing hereditary mutations linked to diseases like sickle cell anemia and muscular dystrophy.
- Applications in Crop Improvement: The technology is also applicable to plant systems, suggesting potential non-transgenic applications in crop improvement.
