Q. The correct order of steps in Polymerase Chain Reaction (PCR) is given in which of the following codes?

[A] Denaturation, Extension, Annealing

[B] Annealing, Extension, Denaturation

[C] Extension, Denaturation, Annealing

[D] Denaturation, Annealing, Extension

Answer: D
Notes:

PCR is based on three simple steps required for any DNA synthesis reaction:

(1) denaturation of the template into single strands;

(2) annealing of primers to each original strand for new strand synthesis; and

(3) extension of the new DNA strands from the primers.

 

 

Source: ForumIAS

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