rt-LAMP Assay for Tuberculosis Diagnosis

News- Researchers have evaluated the real-time Loop-Mediated Isothermal Amplification (rt-LAMP) method for the early and accurate detection of tuberculosis (TB). rt-LAMP Assay for Tuberculosis Diagnosis.

About rt-LAMP Assay

• rt-LAMP (Real-time Loop-mediated Isothermal Amplification) is a molecular diagnostic test that enables the rapid detection of TB DNA.
• It uses a one-step DNA amplification process, capable of detecting TB DNA with as few as 10 copies per microliter in a sample.
• The test functions at a single temperature, unlike traditional RT-PCR which requires three different temperature cycles.
• The rt-LAMP assay has been approved by the Central Drugs Standard Control Organisation (CDSCO) and is currently awaiting validation from the Indian Council of Medical Research (ICMR).

Key Features:

• Open Platform: The rt-LAMP assay is designed as an open platform system, which allows existing RT-PCR machines to be repurposed for TB diagnosis by reprogramming them.
• Primer Design: It uses six primers, significantly more than the two primers used in RT-PCR, enhancing its efficiency and specificity.
• Rapid Results: Thanks to its high amplification rate, positive results can be obtained in just 10–20 minutes.
• Low Cost: The test uses inexpensive dyes like Syto 16 (a fluorescent dye) and affordable primers, making it cost-effective.
• High throughput capability: The rt-LAMP system can process up to 96 or even 384 patient samples in a single run, making it suitable for mass screening.