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News: Researchers at the Jawaharlal Nehru Centre for Advanced Scientific Research developed the CLEAR platform for high-resolution protein mapping.
About Cleavable Light-Erased Antibody Reporter (CLEAR) Platform
- Cleavable Light-Erased Antibody Reporter (CLEAR): It is a novel spatial protein imaging platform that enables visualization and mapping of a large number of proteins within the same sample.
- Developed by: The platform was developed by researchers at Jawaharlal Nehru Centre for Advanced Scientific Research, an autonomous institute of the Department of Science and Technology (DST).
- Working Mechanism:
- Targeted Labelling: Scientists use CLEAR probes containing antibodies linked with fluorescent tags through a light-sensitive chemical bond to label proteins.
- Image Capture: The labelled proteins are imaged under a microscope within the same spectral window.
- Photolytic Erasing: A gentle pulse of 365 nm LED light removes the fluorescent signal by cleaving the chemical bond without damaging the sample.
- Cyclic Rewriting: A new set of proteins is labelled and imaged repeatedly in the same optical window to generate detailed protein maps.
- Detailed Mapping: Repeated imaging cycles create information-rich maps across cells and tissue sections.
- Key Features of CLEAR:
- High-Plex Multiplexing: It enables visualization of a very large number of proteins within a single biological sample.
- Live-Cell Compatibility: The gentle light-erasing process preserves delicate biological samples, including live cells.
- High Spatial Resolution: It captures the precise spatial arrangement of proteins within tissues.
- Speed and Simplified Imaging: The platform repeatedly uses a single fluorescent channel and reduces dependence on complex multi-laser imaging systems.
- Chalkboard-Like Imaging: The platform repeatedly erases and rewrites fluorescent signals like a chalkboard using a single fluorophore.
- Significance: CLEAR can improve disease diagnosis, biomedical research, and precision medicine through comprehensive high-resolution protein mapping.
